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生化所李永泉教授课题组在Applied and Environmental Microbiology 发表论文

时间:2014-12-25 访问次数:1828

Applied and Environmental Microbiology为美国微生物学会会刊,列入浙江大学TOP期刊。该论文第一作者为博士生俞品,通讯作者为李永泉教授。Pin Yu, Shui-ping Liu, Qin-Ting Bu, Zhen-Xing Zhou, Zhen-Hong Zhu, Fang-Liang Huang ,  Yong-Quan Li*,  WblAch, a Pivotal Activator of Natamycin Biosynthesis and Morphological Differentiation in Streptomyces chattanoogensis L10, Is Positively Regulated by AdpAch, Applied and Environmental Microbiology,2014,80(22):6879-87doi: 10.1128/AEM.01849-14doi: 10.1128/AEM.01849-14,

http://aem.asm.org/content/80/22/6879.full.pdf

    论文解析了恰塔努加链霉菌L10whiB家族调控蛋白WblAch的调控机制。研究表明,WblAchadpAch敲除株中表达量下降明显,qRT-PCR实验证实AdpAch正调控wblAch的转录,EMSADNaseI Footprinting 实验表明AdpAch直接结合在wblAch的启动子上;wblAch的缺失导致纳他霉素合成能力丧失,形态分化也受到严重影响,qRT-PCR实验表明whi基因在wblAch菌株中的低表达量可能是形态分化受阻的原因;从而揭示了wblAchAdpAch耦联协同调控菌体细胞的形态分化与纳他霉素生物合成的分子机制。